Coding
Part:BBa_K1491025:Design
Designed by: Danielle Peters Group: iGEM14_Carnegie_Mellon (2014-09-19)
T7 RNA Polymerase with ER Intein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2464
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2889
Illegal AgeI site found at 2853 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2364
Design Notes
Estrogen sensitive T7 RNA polymerase
Source
T7 RNA polymerase is BBa_K145001 and intein is BBa_K1491024